Long non?coding RNA NEAT1 promotes the malignancy of laryngeal squamous cell carcinoma by regulating the microRNA?204?5p/SEMA4B axis

Laryngeal squamous cell carcinoma (LSCC) is a highly invasive malignant tumor in the head and neck area. As an oncogene, long non?coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes proliferation, migration invasion several types of cancer. The present study aimed to reveal effects NEAT1 on progression LSCC. Reverse transcription?quantitative PCR (RT?qPCR) was used detect relative mRNA expression levels NEAT1, microRNA (miR)?204?5p semaphorin (SEMA) 4B. Kaplan?Meier analysis analyze overall survival times. in?situ hybridization (ISH) exhibited distribution miR?204?5p tissues. fluorescence ISH cells. Western blot level target proteins. Cell viability analyzed using MTT assay, while flow cytometry determine apoptosis. Wound healing Transwell assays were value invasion, respectively. immunoprecipitation bioinformatics prediction dual luciferase reporter assay relationship. RT?qPCR results showed that expressed had decreased LSCC tissues cells compared with normal tissue 16HBE?14o line, Knockdown small interfering (si) overexpressed both effectively inhibited Besides, further experiments revealed NEAT1. At same time, silenced reversed anti?tumor si?NEAT1. In addition, SEMA4B targeted by upregulated weakened antitumor regulated targeting Overall, promoted proliferation regulating miR?204?5p/SEMA4B axis.